In the robbery report on the Batcomputer, the Carson Chemicals burglary is misspelled "buaglary".
This episode was the first one to feature Batman bleeding. Bruce Timm stated on the DVD commentary for this episode that he and Paul Dini fought with the censors to allow this shot, which was deemed inappropriate for younger viewers.
Dr. March's line "They're survivors" of referring to bats was similarly said by Batman in Tim Burton's Batman that "They're great survivors".
This marks one of the very few times in the series where Batman speaks using Bruce Wayne's voice, which later happens again in "Heart of Steel Part I".
This episode's opening where Man-Bat's flying through Gotham is reversed shot for shot in the end of the Justice League Unlimited episode "Epilogue" with Terry McGinnis. Also, the blimp pilot (voiced by Kevin Conroy) who asks "Did you see that?" is mirrored by a Gotham police officer (also voiced by Conroy) asking the same question as the last line spoken.
For the Batman Beyond episode "Splicers", Will Friedle was given a tape of this episode to help him prepare for voicing Terry McGinnis's Man-Bat transformation.
The opening of the episode is mirrored in the opening scene of the Supernatural episode "Like A Virgin" with a small plane passenger asking the pilot if they "saw that" flying outside the window that looked like a large bat. Perhaps in respect to this, Batman is mentioned later in the episode.