This was the first episode of the series to feature Batman's past. Only the 1989 movie from Tim Burton, and an episode of the Super Powers Team (the last season of the Super Friends), incidentally also featuring the Scarecrow as main villain, explored the Dark Knight's origins outside comics medium before this episode.
Bullock mentions Batman's refusal to comply with the investigation, saying to Gordon "Zorro here's been holding evidence". Incidentally, the character of Zorro was once of the major influences of Bob Kane to create Batman.
The theme music that plays in this episode's title card is later used in the one in "Fear of Victory".See Music
At one point the Batmobile computer shows a list of nearby chemical companies. Among the companies shown are S.T.A.R. Labs from Superman's comics, which would later be featured in Superman: The Animated Series, and Axis Chemicals, which is the company that turned Jack Napier into the Joker in the 1989 Batman movie.
Near the end of the episode, the people of the university are exposed to fear toxin and attack Batman in fear of him. Later, Batman exposes the Scarecrow to the fear toxin, which causes him to hallucinate that Batman is a monstrous bat-like creature. Similar scenarios later occur in the movie Batman Begins. The former was similarly used in the 1989 movie in which Joker gasses the people of a restaurant to sleep. A much darker and ruthless version of Scarecrow also sees Batman as a more demonic creature at the end of the videogame Batman: Arkham Knight.
The security guard at the bank is reading a Tiny Toon Adventures comic book.
Batman's line "I am vengeance... I am the night... I am BATMAN!" is first used in this episode and it became somewhat iconic, being used by Cartoon Network throughout their advertising campaign when they first began airing Batman: The Animated Series.
It was later used as the opening line of the Batman musical in "Out of the Past".
The line has also become a signature of Kevin Conroy, and he often performs it the start of his appearances at conventions. Conroy later said the line in the 2013 "Batman vs. Superman" episode of The Daly Show, starring Tim Daly and his son, Sam. Conroy also said the line when reprising Batman during Batman: Arkham Knight.
The line is also part of Green Arrow's "Batman impression" in Batman: The Brave and the Bold, in the episodes "Dawn of the Dead Man!" and "Night of the Batmen".