Though Batman: The Animated Series is based primarily on DC Comics, the show introduced several new innovations that were later adapted into their mainstream comics and subsequently other media.
- Salvatore Valestra featured in Justice League of America 80-Page Giant #1 under the alias of Chucky Sol.
- A Batwing identical to the BTAS design is seen in Batman Confidential #36
- In Batman: Shadow Of The Bat #1,000,000, Batman tells his origin story through a tape to a group of villains. He starts with "I am Vengeance". as Batman did in the BTAS episode Nothing to Fear.
- In Nightwing #1, Dick Grayson fights a new villain called Saiko on a rooftop who purposely misses him to hit a water tower to "wash" him off the roof as Kyodai Ken did to him in Day of the Samurai.
- Summer Gleeson appeared in the Black & White Feature of Batman: Gotham Knights #33.
- In Catwoman #2, Selina Kyle arrives at a charity event where Bruce Wayne is the center of attention, with long blonde hair and wearing a red dress to get Bruce's immediate attention as she did in The Cat and the Claw Part I.
- The Batman: The Brave And The Bold Arkham Asylum's exterior looks the same as it does in BTAS.
Several original characters were created for the show that have since featured in DC comic books. Such examples are Harley Quinn, Renee Montoya, Nora Fries and Lock-Up. Even The Condiment King was introduced into the mainstream in Birds Of Prey #37 and dies in Final Crisis Aftermath: Run! #4.However, his real name is "Mitchell Mayo" rather than Buddy Standler.
In a similar way the representations of characters in BTAS have bee adapted to their comic counterparts. This includes Mr. Freeze's tragic past and motivation for crime relating to his wife, Zatanna and Zatara traing Bruce Wayne in his youth, The Riddler wearing a business style suit for a costume rather than a jumpsuit and the re-invention of The Clock King as Temple Fugate rather than the original Green Arrow villain William Tockman.