|Portrayed by:||Melissa Gilbert|
Mary Kay Bergman
|First appearance:||Heart of Steel Part I|
|Last appearance:||Batman & Mr. Freeze: SubZero|
|Appears in:||7 Episodes & 2 Films|
Barbara was first entangled with Batman's affairs when a renegade artificial intelligence machine, H.A.R.D.A.C., tried to replace the most influential citizens of Gotham City, such as James Gordon, Mayor Hamilton Hill, and Detective Harvey Bullock. Barbara was one of the few people who initially felt something had happened. Using a few skills she picked up from her father and her experience a a gymnast while at Gotham University, Barbara managed to help Batman save the city from H.A.R.D.A.C.
Barbara was then seen when her father was shot by Jazzman on the anniversary of the death of Bruce Wayne's parents. A sorrowful Batman visits Gordon in Gotham City Hospital, where Barbara tells him not to blame himself. Batman takes heart and captures Jazzman, while Gordon recovers.
When Commissioner Gordon was framed by Two-Face, Barbara donned a bat costume to help investigate. She did, however, find that the costume fit her better than she had anticipated, as she proved vital in clearing Gordon's name and capturing Two-Face along with his associates. Regardless of whether they agreed or not, Batman and Robin soon realized that they had a new ally in the form of Batgirl.
However, Barbara's studies at the Gotham University prevented her from going out more often as Batgirl until she learned that the Jade Cat Statue was stolen from the University's museum and went to investigate as Batgirl. During the investigation, Batgirl came across Catwoman, who was also looking for the criminal to clear her name and the two of them formed a temporary alliance to find the criminal. The two of them eventually crossed paths with Roland Daggett, who was behind the theft, but the heroines would have perished against Daggett's men if Robin had not arrived in time to assist them. After capturing Daggett, Catwoman revealed her true intentions of stealing the Cat Statue, but Batgirl stopped her and forced her to surrender to the law. However, Catwoman escaped and Batgirl allowed to go free, knowing that there would be more opportunities to capture her.
A short time later, Barbara started a relation with Dick Grayson, but before they could get serious, Barbara was picked as an organ donor to Nora Fries by Mr. Freeze, who kidnapped Barbara to sacrifice her in order to save his wife. Barbara endured as Freeze's prisoner long enough for Batman and Robin to come rescue her. The fight that followed caused Freeze's lair to explode. Batman, Robin, and Barbara were able to evacuate Nora and Freeze's Inuit ward Koonak in time, but Freeze had fallen into the fiery blast and was assumed dead. In actuality, he and his pet polar bears had survived. Back in Gotham, Nora was cured, much to Freeze's delight.
- I Am the Night (as Barbara Gordon)
- Heart of Steel Part I (as Barbara Gordon)
- Heart of Steel Part II (as Barbara Gordon)
- Shadow of the Bat Part I
- Shadow of the Bat Part II
- Batgirl Returns
- The Adventures of Batman & Robin (SNES) (as Barbara Gordon)